Ten days after transfection, a lab at the Technical University of Munich needed to screen single-cell colonies from a 96-well plate to find which clones carried the CRISPR knockout they were looking for.

Conventional genotyping after CRISPR editing can take three to four weeks once cells are expanded enough to extract from reliably. This team used prepGEM Universal directly on the 96-well plate, extracting DNA from each well in a single temperature-driven reaction without transferring cells to separate tubes.

The extracted DNA was suitable for both PCR and Sanger sequencing, allowing the team to identify which clones carried the desired genetic alteration directly from low cell numbers, without the standard multi-week wait for colonies to reach a size conventional extraction methods would need.

Genome editing screening is often described as slow because the editing step is complex. In this case study, the actual constraint downstream was extraction, not the biology.

A workflow that took weeks with standard kits ran in days. If your CRISPR screening timeline is dominated by waiting for extraction, not editing, that is a chemistry problem, and prepGEM Universal already has the fix.